The Capturem His-Tagged Purification Miniprep Kit provides single-use disposable membrane spin columns for simple, rapid purification of his-tagged proteins in up to 800 μl of clarified lysate. The columns are suitable for use under native or denaturing conditions, in the presence of additives such as DTT (up to 10 mM), βME (up to 30 mM), TCEP (up to 5 mM), EDTA (up to 10 mM), or glycerol. 20 purifications